(a) Deconvolved image of SRA-1010 in a single cell acquired at high magnification, XY orientation. Scale bar = 2μm
(b) Superimposed image of the nuclear shell and SRA-1010 bisected in XY. The ER is pseudocolored green, the nucleus blue and receptors that colocalise with the nucleus are white. 6% of the voxels containing SRA-1010 were within the nucleus.
(c) Superimposed image of the nuclear shell and SRA-1010 rotated 90° around the Y-axis. The apical side of the cell is shown by the arrow.
As with the C-terminus antibody the majority of receptors are located outside the nucleus
